Altogether, the observation that SALI cultures express other ATII cell markers (e.g., SPA, SPC) and the ability of H441 cells to model ion transport28 suggest the SALI model could be useful for the study of other diseases of surfactant metabolism (related to SFTPC or ATP-binding cassette sub-family A member 3 [ABCA3] genes) and other ILDs, such as pulmonary alveolar microlithiasis caused by inadequate phosphate clearance53 (related to solute carrier family 34 member 2 [SLC34A2] gene). This evidence concerns the gene ABCA3 and pulmonary alveolar microlithiasis.