Our results demonstrated that EGCG was effective in suppressing the phosphorylation of NF-κB, inhibiting the expression of MDM2, and activating the downstream proteins of Bcl-2 and PARP, and then promoted the apoptosis induction, which might contribute to the synergistic effect of combination with DOX against bladder cancer proliferation and migration. The gene discussed is NFKB1; the disease is urinary bladder cancer.