Furthermore, the expression of PI3K-Akt pathway-related proteins in the S1 and MC38 cells was measured by WB, and the results showed that P. gingivalis 33277 had no effect on p-Akt (308), p-Akt (473) and Akt (pan) levels after 3, 6 and 12 h of infection (Supplementary Figure 3). Here, AKT1 is linked to infection.