With HK2 dissociating from VDAC-1, the integrity and permeability of mitochondria membrane was disrupted which activated a release of pro-apoptotic enzyme, such as cytochrome C. It was well documented in our study that exposure to KM resulted in the decrease of the interaction between HK2 and VDAC-1, which could induce CRC cells to undergo apoptosis. The gene discussed is CYCS; the disease is colorectal carcinoma.