Here, Beclin 1 was demonstrated to suppress proliferation, migration, invasion and tumor growth, and induce cell cycle arrest, apoptosis, autophagy and chemoresistance in gastric cancer cells, whereas versa for Becn1-silencing cells, suggesting that Beclin 1 can be used as a gene therapy target of gastric cancer if its chemoresistant induction could be avoided or alleviated. Here, BECN1 is linked to gastric cancer.