Moreover, the expression of Glypican-3 (a differentiation marker of live cancer cell) in transplanted tumors was significantly decreased with the increase of DOX concentration (74.59% ± 7.46% versus 59.69% ± 4.97%, p = 0.0009767 < 0.01; 59.69% ± 4.97% versus 42.94% ± 2.55%, p = 0.0000063 < 0.01; 42.94% ± 2.55% versus 34.43% ± 3.37%, p = 0.000086 < 0.01; 34.43% ± 3.37% versus 24.23% ± 3.95%, p = 0.000888 < 0.01) (Figure 1H). The gene discussed is GPC3; the disease is cancer.