To determine whether FAM83B regulated cisplatin resistance of ovarian cancer, we overexpressed FAM83B in ovarian cancer cell line COV362 and SK-OV-3, and knocked down FAM83B in the same cells, Q-PCR and western blot were used to determine the FAM83B expression in cells with FAM83B overexpression and knockdown (Fig. 3a). The gene discussed is SACK1B; the disease is ovarian cancer.