Mentionable, this phenomenon was severer in KC mice acinar cells than C mice acinar cells (consist with previous findings in Fig. 7B) and same as our findings before (Fig. 1f), KC mice (KrasG12D mediated hyper-activation of Ras-MAPK pathway) harbor relative high level HSF1 expression in acinar cells compared to C mice (KrasWT mediated normal-activation of Ras-MAPK pathway), to sum up, above results suggested that apart from the activity of EGFR, abnormal Ras-MAPK can also influence HSF1 via unknown mechanism. This evidence concerns the gene HSF1 and keratoconus.