We treated acinar cell clusters from KC mice with EGF (a natural activate legend of EGFR) and erlotinib/KRIBB11 and the results showed that EGFR activation significantly increased the number (ADM formation) and diameter (ADM growth) of ductal-like spheres, and this “sphere/ADM-promoting” ability was suppressed by erlotinib and KRIBB11 (Fig. 6f-h). This evidence concerns the gene EGF and keratoconus.