To characterize the role of mediator proteins in our system, we performed RPA-eGFP displacement experiments with RAD-51 in the presence or absence of his6-MBP-BRC-2 (hereafter referred to as BRC-2), a recombination mediator and ortholog of the breast and ovarian cancer tumor-suppressor protein, BRCA2 (Martin et al., 2005; Petalcorin et al., 2007; Petalcorin et al., 2006) (Figure 1B). Here, RPA1 is linked to ovarian cancer.