VIM and neoplasm: For the Epithelial–Mesenchymal Transition (EMT) is an initial step in the process of tumor metastasis, we detected the epithelial and mesenchymal markers in vitro by immunofluorescence (IF) and WB assays, and N4 markedly lead to the upregulation of E-cadherin, and downregulation of N-cadherin and vimentin in Pan02 cells (Fig. 8A, B).