Cell-free plasma DNA concentrations have been shown to be higher in ICU nonsurvivors and correlate with lactate levels and sequential organ failure assessment scores.49,50 To confirm the implication of TREM-1 in NET release in vivo, we measured both plasma cf-DNA and the cf-DNA associated with MPO (cf-DNA/MPO), which are two major constituents of NET structures, in mice with LPS-induced experimental septic shock. Here, MPO is linked to septic shock.