Treatment of tumor spheroids with NF-κBinhibitors slightly enhanced HLA-A02 expression on cell surface membranes anddecreased IFNg and TNFa secretion, though these phenotypes would not explain thestrong anti-tumor efficacies observed during T-cell/spheroid co-culture treatment.It is tantalizing to speculate that co-culture of T-cells with tumor spheroids mayactivate NF-κB signaling in one of these cell types that is not present inmonoculture and that activation of this paracrine signaling mechanism is responsiblefor the observed efficacies of NF-κB-targeting compounds. The gene discussed is NFKB1; the disease is neoplasm.