PDCD1 and neoplasm: During our study, we revealed a potent abilityof cyclopamine to induce antigen-dependent T-cell-mediated tumor cytotoxicity.Through FACS-based T-cell phenotyping, we observed modest yet significant increasesin both total CD3+ andCD8+/CD25+ cytolyticT-cell proliferation under cyclopamine treatment as well as upregulation of thecheckpoint molecule PD-1.