The third phase of T-cell expansionrequired culturing T-cells in “resting” medium, which contained IL-2, IL-4, andIL-7 (Fig, 2c, day 9) and resulted inthe further expansion of the desiredCD8+CD127+ population.Failure to expand T-cells in a resting medium resulted in aggressive cytolyticbehavior upon exposure to tumor spheroids in a non-antigen-dependent manner(Supplementary Fig. 1c). This evidence concerns the gene IL2 and neoplasm.