For a more detailed analysis of T-cell phenotypes during co-culturewith tumor spheroids under CDK4/6i treatment, we extracted T-cells from thesecultures and profiled them for functional markers of active tumor killing.Nearly all of the CD8+ T-cells were positive for thedegranulation marker CD107 indicating a high degree of tumor killing(Fig. 8b). This evidence concerns the gene CD8A and neoplasm.