PRRT2 and melanoma: The ongoing presence of PKC isoforms following treatment with active epoxytiglianes was not unique to MM649 cells; treatment of SK-MEL-28 melanoma (Fig. 6B) or B16-F0 murine melanoma cells (Fig. 6C) with TT showed maintenance of PKC isoform protein level compared to PMA even with 24 h exposure and 24 h recovery from treatment.