To examine the role of YTHDF2 in GBM tumorigenesis, we used two distinct short hairpin RNAs targeting the 3′UTR (shYTHDF2#1) or the CDS (shYTHDF2#2) of YTHDF2 mRNA to ablate YTHDF2 expression in GSC11 and GSC7-2 cells (Supplementary Fig. 2a). Here, YTHDF2 is linked to glioblastoma.