While FP-RMS and FN-RMS had similar enhancer structure surrounding SNAI2 and transcriptomic levels of SNAI2, there is a selective dependency for SNAI2 among FN-RMS cells when compared to a panel of cancer cell lines in genome-wide CRISPR screening data from the Achilles Project (Fig. S1g, h).Together our data provide evidence that SNAI2 is expressed in RMS, is regulated by a 1.2 Mb regulatory locus to which muscle-specific transcription factor MYOD can bind to maintain its expression and there is a dependency for SNAI2 in FN-RMS cells. This evidence concerns the gene SNAI2 and cancer.