In addition, western blot analysis of CT26 tumor tissues showed that the extracellular and cytoplasmic HMGB1 was significantly enhanced in H@Gd-NCPs+RT (6 Gy × 1) group compared with Saline, RT (6 Gy × 1), Gd-NCPs + RT (6 Gy × 1) groups (Fig. 6e). This evidence concerns the gene HMGB1 and neoplasm.