CD274 and neoplasm: Novel methods such as circulating tumor DNA [68], TCR sequencing on peripheral T cells [69], tumor biopsies immune-profiling analyses by next-generation sequencing and high-throughput flow cytometry/mass cytometry [70], and isotope-labeled PD-1 and PD-L1 targeted imaging [71] will hopefully lead to a more accurate and reliable diagnosis of acquired ICI resistance in the future.