ALDH1B1 and colon adenocarcinoma: To this end, we utilized the human colon adenocarcinoma cell line (HT29) and generated an isogenic HT29 cell line pair (differing only in the expression of human GFP-tagged ALDH1B1 in order to investigate the effect of ALDH1B1 overexpression in (i) cell morphology, (ii) cell proliferation and colony formation efficacy, (iii) cell cycle progression, (iv) cell viability (under conditions of exogenous stresses imposed by treatment with chemotherapeutic agents), (v) gene expression profile of epithelial–mesenchymal transition (EMT) markers and (vi) cell migration.