To identify important regulators of SOST expression presence in three bone-related cells (mesenchymal stem cells—MSC-; human fetal osteoblasts—hFOB- and an osteosarcoma cell line—Saos-2-), we performed a 4C-seq assay using the SOST proximal promoter (chr17:41838135-41838123, GRCh37/hg19) as a viewpoint (Figure 3). Here, SOST is linked to osteosarcoma.