No variants matching our filtering criteria were detected in any known genes associated with Noonan syndrome, other RASopathies (PTPN11, SOS1, RAF1, RIT1, KRAS, NRAS, BRAF, SHOC2, CBL, MEK1, MEK2, HRAS, MAP2K1, MAP2K2, NF1), or other genes associated with CHARGE syndrome (SEMA3E). Here, RAF1 is linked to CHARGE syndrome.