Wild-type (Figure 3a) and MSLN+ HEK293 (Figure 3b) cells were infected with different dosage of THV_SS1 (MOI 0.001, 0.01, 0.1, 1 for HEK293-MSLN; MOI 1 for HEK293), and viral spread was monitored until reaching the infection of the whole cell monolayer. This evidence concerns the gene MSLN and infection.