IDH1 and central nervous system cancer: In the present study, we established a novel assay that applies liquid biopsy targeting the ctDNA in CSF using chip-based digital PCR (dPCR) for detecting the glioma-specific diagnostic driver mutations such as IDH1 R132H (IDH1), H3F3A K27M (H3 K27M), and TERT promoter (pTERT) mutations.