YAP1 and cancer: To further validate that LATS2 was a direct target of miR-429 and determine whether miR-429 suppresses cancer cell proliferation and growth by regulating LATS2 and then YAP/TAZ expression, flag-tagged LATS1 and LATS2 overexpressing plasmids were constructed and transfected into RKO or HCT116 cell line together with miR-429 mimics.