To determine whether these rare variants of TENM4 contribute collectively to sporadic PD risk in our cohort, we performed a gene-based burden analysis using Fisher’s exact test (Nicolae, 2016), and a clear general enrichment was detected for early onset Parkinson’s disease (EOPD, age at onset ≤ 50) patients (p < 0.001, OR = 5.264, 95% CI = 1.957–14.158). This evidence concerns the gene TENM4 and Parkinson disease.