In order to determine whether Sch B-mediated osteosarcoma progression regulated by circ_0009112 and miR-708-5p was related to PI3K/AKT pathway, the effects among Sch B, circ_0009112 and miR-708-5p on protein expression of PI3K, p-PI3K, AKT and p-AKT were revealed by western blot. The gene discussed is AKT1; the disease is osteosarcoma.