NLRP3 and infection: To study inflammatory response of Brucella infection in vitro, macrophages were infected by S2308 at different time and NLRP3 and Pro-caspase-1 were detected at mRNA (Figures 1A, B) and protein levels (Figure 1C), our results showed that inflammasome NLRP3 and Pro-caspase-1 were significantly increased, especially at 24 h of infection.