AAV9-CTRP5, AMPKα2 global knock out (AMPKα2−/−)and AAV9-CTRP5+ AMPKα2−/− mice were used to establish cardiac I/R or infarction associated HF models to investigate the roles and mechanisms of CTRP5 in vivo. This evidence concerns the gene PRKAA2 and hydrops fetalis.