The ELISA data indicate that CVB3 elevated the levels of the inflammatory cytokines TNF-α, IL-6, and IL-1β in H9c2 cells, and the qRT-PCR data further revealed that CVB3 promoted the mRNA expression of the fibrosis-related genes fibronectin, collagen I, and collagen III in H9c2 cells, suggesting the successful establishment of CVB3-induced myocarditis (Figures 1(e) and 1(f), P < 0.05). This evidence concerns the gene TNF and myocarditis.