Specifically, the pre-castrated LTL-331 PDX initially exhibited a classic adenocarcinoma phenotype and gradually transformed to the t-NEPC LTL-331R PDX after surgical castration, during the process EHF was notably depressed and positively associated with AR, and negatively with SYP and SOX2 (Fig. 1D). The gene discussed is SYP; the disease is adenocarcinoma.