Specifically, the pre-castrated LTL-331 PDX initially exhibited a classic adenocarcinoma phenotype and gradually transformed to the t-NEPC LTL-331R PDX after surgical castration, during the process EHF was notably depressed and positively associated with AR, and negatively with SYP and SOX2 (Fig. 1D). Here, SOX2 is linked to adenocarcinoma.