EHF and cancer: We then tested EHF expression in a panel of five PCa cell lines and found that EHF was highly expressed in the LNCaP, C4-2, and 22Rv1 AdPC cell lines, but relatively depressed in the androgen-insensitive DU145 cells as well as the PC-327 and NCI-H660 NEPC cell lines (Fig. 1E), which was consistent with the microarray data from these cancer cells reported by Barretina et al. 24 (Fig. S1b).