Using PI/FITC flow cytometry, we found that nutlin3 can induce apoptosis in p53 wild-type renal cell carcinoma cells (Fig. 3D, E), but knocking down p53BER2 does not reverse nutlin3-mediated apoptotic response in ACHN (Fig. 3D) and OSRC-2 (Supplementary Fig. 2E). The gene discussed is TP53; the disease is hereditary clear cell renal cell carcinoma.