Although no statistically significant findings were obtained when comparing patients with and without childhood trauma, probably due to the reduced sample size, several interesting associations were observed, including regions in the genes NKX2-1, PXDN and POU5F1. Interestingly, POU5F1 was also found differentially methylated in this reduced sample, with BPD patients with childhood trauma presenting lower levels than those without trauma, thus replicating the previous finding. The gene discussed is POU5F1; the disease is Borderline personality disorder.