To validate the role of type I IFN and TNF-α on tumor-infiltrating MDSCs, CD11b+ myeloid cells from fresh CRC tissues were treated with IFN-α/β and TNF-α, and then cocultured with 5 (6)-Carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled T cells. The gene discussed is TNF; the disease is colorectal carcinoma.