In our study, the IC50 ratio ( IC50 against (p53 − / −) HCT 116 to IC50 against (p53 + / +) HCT 116)) clearly show that p53 knockout HCT116 cells were about four times more sensitive to β-carotene than p53 wild type HCT 116 cells, suggesting the use of this compound against colon cancers cells like HCT 116 bearing p53 mutations. Here, TP53 is linked to malignant colon neoplasm.