To further demonstrate the role of PL/miR-34b-3p/TGFBR1 axis in LC cells, we constructed an expression vector of TGFBR1 lacking the 3 ‘UTR (named pcDNA3.1-TGFBR1) which was transfected into PL-treated A549 and H1299 cells (combination group). The gene discussed is TGFBR1; the disease is laryngotracheoesophageal cleft.