We found that TOP2A/MCM2, p16INK4a, cyclin-E, Ki-67, and telomerase increased according to lesion severity, and these observations coincide with other studies that reported biomarkers associated with the development of premalignant lesions and proposed its usefulness to identify the lesions that are most likely to progress to high-grade cervical disease and CC [31]. Here, TOP2A is linked to cervicitis.