However, the supplementation promotes a further increase (+ 35%) of cholesterol in the liver, in comparison with CF mice without supplementation, which is due to the above mentioned alterations in the CF liver, which further increases the inflammatory reaction, supported by the considerable gene expression of TNFα. Cholesterol supplementation has no effect on LDLR, HMG-CoAR, ACAT2 and CYP7A1 expression due to mechanisms to be investigated yet. The gene discussed is ACAT2; the disease is cystic fibrosis.