CD40LG and infection: The DENV-seropositive group was stratified further based on DENV NS1/IgM/IgG serology and the presence of circulating viral RNA: 22/29 samples were DENV RT-PCR positive with the exceptions as indicated (Fig. 5b) and the majority of infections presented as RT-PCR, NS1 and/or IgM positive and thus in the early stages of infection.