APP and Alzheimer disease: Next, to understand heterogeneity in organization of amyloidogenic machinery at finer spatial scales, we performed STED microscopy on one set of age- and gender-matched AD and control samples to investigate if we could enhance the spatial separation between EZ and PSD, and performed an extensive analysis of the nanodomains of β-secretase and APP associated with these functional zones (Figures S9A, S9B, S9C, and S9D and Table S4).