In the current study, we constructed MKN-45 and BGC-823 cells stably expressing mRFP-GFP-LC3 and showed increased accumulation of yellow LC3-II puncta in DSF/Cu-treated cells, indicating increased autophagic flux, which is consistent with the findings reported for non-small cell lung cancer (36). Here, MAP1LC3A is linked to non-small cell lung carcinoma.