To further assess the role of SOX2 in glioma, we silenced SOX2 expression in LN229 and T98G cells by shRNA (Figure S3H) that efficiently suppressed the proliferation and colony formation capability induced by collagen/FN (Figure 3H-J and Figure S3I-K), indicating that SOX2 served as the downstream molecule of PI3K/AKT to induce glioma cell proliferation. The gene discussed is AKT1; the disease is glioma.