CD274 and neoplasm: Loading the PD-L1 trap plasmid into a lipid-protamine-DNA nanoparticle enhanced the local level of PD-L1 trap in tumor microenvironment (TME) but did not induce the appearance of Th17 cells in spleens, indicating that this formulation was better tolerated and had a lower tendency to induce irAEs than the unmodified plasmid (45).