PPBP and infection: In this research we identified and characterized (a) a mixed infection of helenium virus S (HelVS) and two distinct isolates of butterbur mosaic virus (ButMV), one of which lacks a major portion of the “essential” TGB1 gene; (b) identified single infection of HelVS in other cultivars of veronica, established the near-complete genome of this isolate by next-generation sequencing, and confirmed it by Sanger sequencing.