Univariate analysis demonstrated that high SPC25 mRNA level (P < 0.001), Age (P=0.044), M-stage (P < 0.001), T-stage (P < 0.001), and Pathologic stage (P < 0.001) were independent prognostic factors for HCC patients (Figure 3). This evidence concerns the gene SPC25 and hepatocellular carcinoma.