Herein, we combined biochemical, electrophysiological, immunohistological, transcriptomic, behavioral approaches and in vivo brain imaging to assess the hippocampal functions in a mouse model where an ALS-linked human FUS mutation (R514G-FUS) transgene was expressed in the CNS using a murine prion promoter [34]. Here, FUS is linked to amyotrophic lateral sclerosis.