To know the association between liver fibrosis driven by HSCs and intracellular amount of retinol in HSCs, we isolated primary HSCs from Lrat-specific Tif1γ knock-out transgenic mice (Lrat:Cas9-ERT2: sgTif1γ) [16] and cultured them for 3 days in the presence of 10 nM tamoxifen (TMX) to reduce TIF1γ levels, a negative regulator against fibrosis. Here, MAPK3 is linked to Hepatic fibrosis.