We validated the interaction between PTEN and the cell-cycle-related proteins CDK4 and Cyclin D1 with Co-IP and revealed that lnc-GAN1 overexpression increased PTEN level and decreased CDK4 and cyclin D1 levels in NSCLC cell lines, whereas lnc-GAN1 knockdown had the opposite effects. This evidence concerns the gene CDK4 and non-small cell lung carcinoma.