To elucidate whether lnc-GAN1 acted via the miR-26a-5p/PTEN pathway in NSCLC, we performed RNA-seq in lnc-GAN1 overexpressing cells and found that lnc-GAN1 significantly affected the expression of cell-cycle-related genes, such as PTEN. The gene discussed is PTEN; the disease is non-small cell lung carcinoma.