To confirm the tumor suppressor role of lnc-GAN1 in vitro and in vivo, we first examined lnc-GAN1 levels in 7 NSCLC cell lines by qRT-PCR and found that 3 cell lines including A549, H460 and SPC-A1 cells had lower lnc-GAN1 level than the immortalized lung cell line Beas-2B, which is basically consistent with the result in NSCLC tissues, whereas another 4 cell lines including H1975, H1299, 95D and H1650 cells had higher levels (Fig. 2a). This evidence concerns the gene GAN and neoplasm.