To directly verify the role of miR-375 in the regulation of the CTGF-EGFR pathway in gliomas, the impact of CTGF or EGF-induced EGFR stimulation on the expression of proteins involved in CTGF-EGFR signalling was evaluated by western blot in miR-375-overexpressing U87, U251, and G15 cells. Here, EGF is linked to glioma.