AIF1 and Alzheimer disease: To determine the dynamic changes of microglial activation across the lifespan of WT and AD mice, we compared immunofluorescent staining for Iba-1 in cortical and hippocampal regions as well as the spinal cord of WT mice at 3-, 12-, and 24-month-old WT and APP/PS1 mice at 3 and 12 months of age.