The expression levels of TLR4, LYN, PI3K, Akt, NF-kB, TNF-α, IL-1, IL-6, and caspase-3 in the control and control + BBR groups were similar (P > 0.05, Fig. 5), suggesting that BBR exerted minor side effects on PCOS. Here, TNF is linked to polycystic ovary syndrome.