However, in PCOS rats (rats in PCOS-ND and PCOS-HFD groups), these levels were significantly higher (P < 0.05, Fig. 5); compared with the PCOS-ND group, the expression levels of TLR4, LYN, PI3K, NF-kB, TNF-α, IL-1, and IL-6 in the PCOS-HFD group were significantly higher (P < 0.05, Fig. 5). Here, TLR4 is linked to polycystic ovary syndrome.