5e). In order to confirm tumor-specific memory response, tumor-free mice “cured” by the treatment of Nano-FdUMP/Nano-Folox were rechallenged with 4 T1 and CT26-FL3 cells (Fig. S7). Results showed that 4 T1 breast tumor growth was not affected, while CT26-FL3 tumor growth was significantly inhibited in same animals (Fig. S7A). These results confirmed that the combination approach promises for the induction of tumor-specific memory response against CRC, resulting in long-term survival in orthotopic CRC mice (fig. 5c). This evidence concerns the gene DDX53 and neoplasm.